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ARIH2 (NM_006321) Human Over-expression Lysate

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SKU
LY416714
Transient overexpression lysate of ariadne homolog 2 (Drosophila) (ARIH2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201846
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006321, NP_006312
Synonyms ARI2; TRIAD1
Predicted MW 57.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006312
Locus ID 10425
Cytogenetics 3p21.31
Protein Families WB
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Other "ARIH2" proteins (3)
SKU Description Size Price
PH301846 ARIH2 MS Standard C13 and N15-labeled recombinant protein (NP_006312) 10 ug
$3,360.00
LC416714 Lyophilized ARIH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301846 Recombinant protein of human ariadne homolog 2 (Drosophila) (ARIH2), 20 µg 20 ug
$737.00

file-alt Citations

E6AP/UBE3A catalyzes encephalomyocarditis virus 3C protease polyubiquitylation and promotes its concentration reduction in virus-infected cells
Carmody, Notarianni, Sambel et al
Biochem. Biophys. Res. Commun. (2017)
AI Summary: OriGene’s pTruORF plasmids containing the mouse ARIH1 and ARIH2 E3 ligase-encoding sequences were used as PCR templates to produce constructs for functional studies of these proteins in the regulation of EMCV 3C protease ubiquitylation.
Snippet: GST-ARIH1 and pT7CFE1-GST-ARIH2, the mouse ARIH1 and ARIH2 E3 ligase-encoding sequences (NM_019927 and NM_011790, respectively) were amplified by PCR from the pTruORF plasmids containing the ARIH1- or ARIH2-encoding sequence (Origene) using;... GST-ARIH1 and pT7CFE1-GST-ARIH2, the mouse ARIH1 and ARIH2 E3 ligase-encoding sequences (NM_019927 and NM_011790, respectively) were amplified by PCR from the pTruORF plasmids containing the ARIH1- or ARIH2-encoding sequence (Origene) using ...;... GST-ARIH1 and pT7CFE1-GST-ARIH2, the mouse ARIH1 and ARIH2 E3 ligase-encoding sequences (NM_019927 and NM_011790, respectively) were amplified by PCR from the pTruORF plasmids containing the ARIH1- or ARIH2-encoding sequence (Origene) using
Showing 1-1 of 1 papers.
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