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Bim (BCL2L11) (NM_006538) Human Over-expression Lysate

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SKU
LY416580
Transient overexpression lysate of BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 6 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212528
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006538, NP_006529
Synonyms BAM; BIM; BOD
Predicted MW 15.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006529
Locus ID 10018
Cytogenetics 2q13
Protein Families WB
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Other "Bim" proteins (1)
SKU Description Size Price
LC416580 Lyophilized BCL2L11 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Using CETSA assay and a mathematical model to reveal dual Bcl-2/Mcl-1 inhibition and on-target mechanism for ABT-199 and S1
Guo, Song, Xue et al
Eur J Pharm Sci (2020) 142, 105105 DOI: 10.1016/j.ejps.2019.105105
Cited by 12
AI Summary: OriGene products contributed to this study by providing the Bcl-2 (Cat# TP300904) and Mcl-1 (Cat# TP301905) recombinant proteins used in the CETSA assays to assess drug-protein binding and target engagement.
Using CETSA assay and a mathematical model to reveal dual Bcl-2/Mcl-1 inhibition and on-target mechanism for ABT-199 and S1
Guo, Song, Xue et al
Eur J Pharm Sci (2019) 142, 105105 DOI: 10.1016/j.ejps.2019.105105
AI Summary: OriGene products Bim (LY416580), Puma (TP325345), Bax (RC204368), and Bak (RC201216) were used in this study to investigate the roles of pro-apoptotic proteins in validating the on-target effects of BH3 mimetic drugs on Bcl-2 family proteins.
Snippet: Recombinant human Bcl-2 and Mcl-1 were obtained as our report described (Zhang et al., 2011), Bim (LY416580), Puma (TP325345), Bax (RC204368), Bak (RC201216) were purchased from OriGene (Beijing, China). Immunoblotting;- Recombinant human Bcl-2 and Mcl-1 were obtained as our report described (Zhang et al., 2011), Bim (LY416580), Puma (TP325345), Bax (RC204368), Bak (RC201216) were purchased from OriGene (Beijing, China). …
Showing 1-2 of 2 papers.
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