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SF3B3 (NM_012426) Human Over-expression Lysate

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SKU
LY415767
Transient overexpression lysate of splicing factor 3b, subunit 3, 130kDa (SF3B3) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209509
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_012426, NP_036558
Synonyms RSE1; SAP130; SF3b130; STAF130
Predicted MW 135.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_036558
Locus ID 23450
Cytogenetics 16q22.1
Protein Families WB
Protein Pathways Spliceosome
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Other "SF3B3" proteins (3)
SKU Description Size Price
PH309509 SF3B3 MS Standard C13 and N15-labeled recombinant protein (NP_036558) 10 ug
$3,360.00
LC415767 Lyophilized SF3B3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309509 Recombinant protein of human splicing factor 3b, subunit 3, 130kDa (SF3B3), 20 µg 20 ug
$867.00

file-alt Citations

Jerantinine A induces tumor-specific cell death through modulation of splicing factor 3b subunit 1 (SF3B1)
Chung, Tan, Raja et al
Sci Rep (2017) 7, 42504 DOI: 10.1038/srep42504
AI Summary: OriGene products contributed to this study by providing the SF3B1 and SF3B3 expression constructs, which were used to investigate the role of these splicing factors in Jerantinine A-induced tumor-specific cell death.
Snippet: Gene expression data were normalized to GAPDH as the house-keeping gene. The specific primers are shown in Supplementary Table S7. Transfection. The SF3B1 and SF3B3 expression construct was obtained from OriGene Technologies (Rockville, MD, USA). ... All 3 versions Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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