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SNAPIN (NM_012437) Human Over-expression Lysate

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SKU
LY415762
Transient overexpression lysate of SNAP-associated protein (SNAPIN) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202602
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_012437, NP_036569
Synonyms BLOC1S7; BLOS7; BORCS3; SNAPAP
Predicted MW 14.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_036569
Locus ID 23557
Cytogenetics 1q21.3
Protein Families WB
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Other "SNAPIN" proteins (3)
SKU Description Size Price
PH302602 SNAPIN MS Standard C13 and N15-labeled recombinant protein (NP_036569) 10 ug
$3,360.00
LC415762 Lyophilized SNAPIN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302602 Recombinant protein of human SNAP-associated protein (SNAPIN), 20 µg 20 ug
$867.00

file-alt Citations

Snapin mediates incretin action and augments glucose-dependent insulin secretion
Song, Seshadri, Ashraf et al
Cell Metab. (2011) 13 (3), 308-19 DOI: 10.1016/j.cmet.2011.02.002
Cited by 117
AI Summary: OriGene products contributed to this paper by providing snapin-specific shRNA lentivirus (OriGene, TL514505) for snapin knockdown experiments, enabling the authors to assess snapin's role in insulin secretion.
Snippet: E). Snapin Is Critical for Insulin Secretion. To evaluate whether snapin participates in regulation of insulin secretion, we knocked down snapin using murine sequence-specific shRNA lentivirus in cultured mouse islets. Knockdown
Showing 1-1 of 1 papers.
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