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Cytochrome b c1 complex subunit 9 (UQCR10) (NM_013387) Human Over-expression Lysate

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SKU
LY415610
Transient overexpression lysate of ubiquinol-cytochrome c reductase, complex III subunit X (UQCR10), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Other products for "Cytochrome b c1 complex subunit 9 or NM_013387"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202816
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_013387, NP_037519
Synonyms HSPC051; HSPC119; HSPC151; QCR9; UCCR7.2; UCRC
Predicted MW 7.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_037519
Locus ID 29796
Cytogenetics 22q12.2
Protein Families WB
Protein Pathways Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
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Other "Cytochrome b c1 complex subunit 9" proteins (1)
SKU Description Size Price
LC415610 Lyophilized UQCR10 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Mechanism of super-assembly of respiratory complexes III and IV
Cogliati, Calvo, Loureiro et al
Nature (2016) 539 (7630), 579-582 DOI: 10.1038/nature20157
Cited by 228
AI Summary: OriGene's anti-UQCR10 antibody (NM_013387) was used in this study to detect and analyze the presence of the UQCR10 subunit, contributing to the investigation of respiratory complex super-assembly mechanisms.
Snippet: anti-COX5B policlonal, Proteintech (11418- 2-AP);anti-COX1, Invitrogen monoclonal (35-8100);anti-UQCR10, Origene (NM_013387);anti-NDUFS3 monoclonal Abcam (AB14711)
WB
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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