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KCNIP1 (NM_014592) Human Over-expression Lysate

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SKU
LY415191
Transient overexpression lysate of Kv channel interacting protein 1 (KCNIP1), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208255
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014592, NP_055407
Synonyms KCHIP1; VABP
Predicted MW 25.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_055407
Locus ID 30820
Cytogenetics 5q35.1
Protein Families WB
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Other "KCNIP1" proteins (3)
SKU Description Size Price
PH308255 KCNIP1 MS Standard C13 and N15-labeled recombinant protein (NP_055407) 10 ug
$3,360.00
LC415191 Lyophilized KCNIP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308255 Recombinant protein of human Kv channel interacting protein 1 (KCNIP1), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

Synthesis by native chemical ligation and characterization of the scorpion toxin AmmTx3
Zoukimian, Meudal, De Waard et al
Bioorg. Med. Chem. (2019) 27 (1), 247-253 DOI: 10.1016/j.bmc.2018.12.009
AI Summary: OriGene contributed to this study by providing the human pCMV6-Entry-KCNIP1 (NM_014592) construct, which was used in the characterization of the scorpion toxin AmmTx3.
Snippet: Louis) and was previously described. 27 The human pCMV6-Entry-KCNIP1 (NM_014592) construct was purchased from Origene (Origene Technologies, Rockville, USA). The human pRC/RSV-DPP6S construct was a generous gift from Dr. Keiji Wada
Showing 1-1 of 1 papers.
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