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ZNF516 (NM_014643) Human Over-expression Lysate

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SKU
LY415147
Transient overexpression lysate of zinc finger protein 516 (ZNF516) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221220
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014643, NP_055458
Synonyms HsT287
Predicted MW 124.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_055458
Locus ID 9658
Cytogenetics 18q23
Protein Families WB
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Other "ZNF516" proteins (1)
SKU Description Size Price
LC415147 Lyophilized ZNF516 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Replication stress links structural and numerical cancer chromosomal instability
Burrell, McClelland, Endesfelder et al
Nature (2013) 494 (7438), 492-6 DOI: 10.1038/nature11935
Cited by 924
AI Summary: In this study, OriGene products—including shRNA lentiviral particles targeting PIGN, MEX3C, and ZNF516—were used to silence these CIN-suppressor genes, enabling the authors to investigate their roles in replication stress and chromosomal instability in colorectal cancer cells.
Snippet: Cancer chromosomal instability (CIN) results in an increased rate of change of chromosome number and structure and generates intratumour heterogeneity. CIN is observed in most solid tumours and is associated with both poor prognosis and drug resistance. Understanding a
Showing 1-1 of 1 papers.
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