ST18 (NM_014682) Human Over-expression Lysate
SKU
LY415116
Transient overexpression lysate of suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) (ST18) (as WB positive control)
$665.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC215211 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_014682, NP_055497 |
| Synonyms | NZF-3; NZF3; ZC2H2C3; ZC2HC10; ZNF387 |
| Predicted MW | 115.2 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_055497 |
| Locus ID | 9705 |
| Cytogenetics | 8q11.23 |
| Protein Families | WB |
Reviews
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Other "ST18" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC415116 | Lyophilized ST18 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
ST18 Enhances PV-IgG-Induced Loss of Keratinocyte Cohesion in Parallel to Increased ERK Activation
Radeva, Walter, Stach et al
Front Immunol (2019) 10, 770 DOI: 10.3389/fimmu.2019.00770
Radeva, Walter, Stach et al
Front Immunol (2019) 10, 770 DOI: 10.3389/fimmu.2019.00770
AI Summary: OriGene's pCMV6-ST18-Myc/DDK construct and pCMV6-Entry vector were used to overexpress ST18 and serve as control, respectively, enabling the study of ST18's role in keratinocyte cohesion and ERK activation in response to pemphigus autoantibodies.
Snippet: The human ST18 construct (pCMV6-ST18-Myc/DDK) was generated by inserting the translated region of a human ST18 cDNA into the pCMV6-Entry target vector (Origene Technologies Company, Rockville, MD, USA). The latter was used also for a control transfection
Showing 1-1 of 1 papers.
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