CHSY1 (NM_014918) Human Over-expression Lysate
SKU
LY414926
Transient overexpression lysate of chondroitin sulfate synthase 1 (CHSY1) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207899 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_014918, NP_055733 |
| Synonyms | CHSY; ChSy-1; CSS1; TPBS |
| Predicted MW | 91.8 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_055733 |
| Locus ID | 22856 |
| Cytogenetics | 15q26.3 |
| Protein Families | WB |
| Protein Pathways | Chondroitin sulfate biosynthesis, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CHSY1" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC414926 | Lyophilized CHSY1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability
Liao, Liao, Tseng et al
Oncogenesis (2020) 9 (2), 9 DOI: 10.1038/s41389-020-0197-0
Liao, Liao, Tseng et al
Oncogenesis (2020) 9 (2), 9 DOI: 10.1038/s41389-020-0197-0
AI Summary: In this study, OriGene products—including the CHSY1 Human cDNA ORF Clone (Cat# RC209347) and CHSY1 shRNA plasmids (Cat# TL309347)—were used to overexpress and silence CHSY1 in glioma cells, enabling the authors to investigate CHSY1’s role in glioblastoma proliferation and PDGFRA signaling.
Snippet: key to improving their treatment. In this study, we showed that CHSY1 is not only a prognostic factor for poor survival of glioma patients but that it also activates the PDGFRA pathway to promote tumorigenesis. In addition to its potential as a therapeutic target in GBM, CHSY1 may help to identify tumors that rely on PDGF signaling. Materials and methods Cell culture and tranfection The source a
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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