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SASH1 (NM_015278) Human Over-expression Lysate

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SKU
LY414668
Transient overexpression lysate of SAM and SH3 domain containing 1 (SASH1) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218866
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015278, NP_056093
Synonyms CAPOK; dJ323M4.1; DUH1; SH3D6A
Predicted MW 136.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_056093
Locus ID 23328
Cytogenetics 6q24.3-q25.1
Protein Families WB
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Other "SASH1" proteins (1)
SKU Description Size Price
LC414668 Lyophilized SASH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Activation and cleavage of SASH1 by caspase-3 mediates an apoptotic response
Burgess, Bolderson, Adams et al
Cell Death Dis (2016) 7 (11), e2469 DOI: 10.1038/cddis.2016.364
AI Summary: OriGene’s PCMV6 mammalian expression vector was used to clone and overexpress the SASH1 gene, enabling the researchers to study the effects of SASH1 and its cleavage fragments on apoptosis in this study.
Snippet: SASH1 fragment overexpression was assessed by western blot analysis.SASH1 overexpression. The SASH1 gene was cloned into a mammalian expression vectorPCMV6 (Origene, Dianostic Technology;Belrose NSW, Australia). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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