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SLC39A9 (NM_018375) Human Over-expression Lysate

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SKU
LY413070
Transient overexpression lysate of solute carrier family 39 (zinc transporter), member 9 (SLC39A9) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209523
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018375, NP_060845
Synonyms ZIP-9; ZIP9
Predicted MW 32.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_060845
Locus ID 55334
Cytogenetics 14q24.1
Protein Families WB
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Documents
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Other "SLC39A9" proteins (1)
SKU Description Size Price
LC413070 Lyophilized SLC39A9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Expression of the zinc importer protein ZIP9/SLC39A9 in glioblastoma cells affects phosphorylation states of p53 and GSK-3β and causes increased cell migration
Münnich, Wernhart, Hogstrand et al
Biometals (2016)
Cited by 19
AI Summary: OriGene contributed to this study by providing the human ZIP9 expression construct, which was used to investigate the role of ZIP9 in glioblastoma cell migration and signaling.
Snippet: Materials and methods. DNA. The human ZIP9 expression construct was purchased from Origene Technologies (CA, USA) and the CMV-GFP construct from Takara/Clontech (Saint-Germain-en-Laye, France). Cell culture. Human ... Cite SaveSaving
Showing 1-1 of 1 papers.
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