Choline dehydrogenase (CHDH) (NM_018397) Human Over-expression Lysate
SKU
LY413062
Transient overexpression lysate of choline dehydrogenase (CHDH) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC205552 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_018397, NP_060867 |
| Predicted MW | 65.4 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_060867 |
| Locus ID | 55349 |
| Cytogenetics | 3p21.1 |
| Protein Families | WB |
| Protein Pathways | Glycine, serine and threonine metabolism |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Choline dehydrogenase" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC413062 | Lyophilized CHDH HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
Accumulation of Betaine in the Developing Mouse Oocyte Requires Choline Dehydrogenase
McClatchie
Thesis (2018)
McClatchie
Thesis (2018)
Snippet: This antibody gave a single band at the predicted molecular size of ~66 kDa. Initial screening for specificity was performed using Human HEK293T transfected with human CHDH cDNA (LY413062, Origene, Rockville, ML) and therefore overexpressing CHDH protein, and an empty vector transfected control cell lysate (LY413062, Origene) as positive and negative antibody controls respectively
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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