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GDAP1 (NM_018972) Human Over-expression Lysate

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SKU
LY412818
Transient overexpression lysate of ganglioside-induced differentiation-associated protein 1 (GDAP1), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216781
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018972, NP_061845
Synonyms CMT4; CMT4A; CMTRIA
Predicted MW 41.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_061845
Locus ID 54332
Cytogenetics 8q21.11
Protein Families WB
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Other "GDAP1" proteins (3)
SKU Description Size Price
LC412818 Lyophilized GDAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420839 Lyophilized GDAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY420839 Transient overexpression lysate of ganglioside-induced differentiation-associated protein 1 (GDAP1), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

Pathogenic Effect of GDAP1 Gene Mutations in a Yeast Model
Rzepnikowska, Kaminska, Kabzińska et al
Genes (Basel) (2020) 11 (3) DOI: 10.3390/genes11030310
Cited by 16
AI Summary: OriGene’s pCMV6-XL5 vector containing GDAP1 cDNA (NM_018972) was used as the template to generate wild-type and mutant GDAP1 constructs, enabling the study of their pathogenic effects in a yeast model.
Snippet: listed in Table 1. The p425-P TDH3 -GDAP1 and p425-P TDH3 -GDAP1m1 plasmids were produced by amplification of full length or truncated GDAP1 cDNA by PCR using a commercially available pCMV6-XL5 vector containing cDNA of GDAP1 (NM_018972;OriGene Cached
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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