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NDUFV2 (NM_021074) Human Over-expression Lysate

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SKU
LY412099
Transient overexpression lysate of NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa (NDUFV2), nuclear gene encoding mitochondrial protein (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200653
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_021074, NP_066552
Synonyms CI-24k; MC1DN7
Predicted MW 27.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_066552
Locus ID 4729
Cytogenetics 18p11.22
Protein Families WB
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
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Other "NDUFV2" proteins (3)
SKU Description Size Price
PH300653 NDUFV2 MS Standard C13 and N15-labeled recombinant protein (NP_066552) 10 ug
$3,360.00
LC412099 Lyophilized NDUFV2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300653 Recombinant protein of human NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa (NDUFV2), nuclear gene encoding mitochondrial protein, 20 µg 20 ug
$867.00

file-alt Citations

Sex-specific genetic regulation of adipose mitochondria and metabolic syndrome by Ndufv2
Chella Krishnan, Vergnes, Acín-Pérez et al
Nat Metab (2021) 3 (11), 1552-1568 DOI: 10.1038/s42255-021-00481-w
Cited by 62
AI Summary: OriGene's NDUFV2-Lentivirus was used to transduce cells for Ndufv2 overexpression studies, enabling the authors to investigate its role in regulating adipose mitochondrial function and metabolic traits.
Snippet: Abstract We have previously suggested a central role for mitochondria in the observed sex differences in metabolic traits. However, the mechanisms by which sex differences affect adipose mitochondrial function and metabolic syndrome are unclear. Here we that , Save;cells were transduced with either a control or NDUFV2-Lentivirus (Origene) and selected with 2.5 µg ml−1 puromycin
Showing 1-1 of 1 papers.
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