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MPST (NM_021126) Human Over-expression Lysate

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SKU
LY412074
Transient overexpression lysate of mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205101
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_021126, NP_066949
Synonyms MST; TST2; TUM1
Predicted MW 33.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_066949
Locus ID 4357
Cytogenetics 22q12.3
Protein Families WB
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
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Other "MPST" proteins (15)
SKU Description Size Price
PH302466 MPST MS Standard C13 and N15-labeled recombinant protein (NP_001013454) 10 ug
$3,360.00
PH305101 MPST MS Standard C13 and N15-labeled recombinant protein (NP_066949) 10 ug
$3,360.00
PH324963 MPST MS Standard C13 and N15-labeled recombinant protein (NP_001013458) 10 ug
$3,360.00
PH325408 MPST MS Standard C13 and N15-labeled recombinant protein (NP_001123989) 10 ug
$3,360.00
LC412074 Lyophilized MPST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422973 Lyophilized MPST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422977 Lyophilized MPST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427224 Lyophilized MPST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422973 Transient overexpression lysate of mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 2 (as WB positive control) 100 ug
$436.00
LY422977 Transient overexpression lysate of mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control) 100 ug
$436.00
LY427224 Transient overexpression lysate of mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 3 (as WB positive control) 100 ug
$436.00
TP302466 Recombinant protein of human mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 2, 20 µg 20 ug
$737.00
TP305101 Recombinant protein of human mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 1, 20 µg 20 ug
$737.00
TP324963 Recombinant protein of human mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 1, 20 µg 20 ug
$737.00
TP325408 Recombinant protein of human mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 3, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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