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KCNK10 (NM_021161) Human Over-expression Lysate

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SKU
LY412052
Transient overexpression lysate of potassium channel, subfamily K, member 10 (KCNK10), transcript variant 1 (as WB positive control)
  • LY412052 is the same product as LY429648.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222775
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_021161, NP_066984
Synonyms K2p10.1; PPP1R97; TREK-2; TREK2
Predicted MW 59.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_066984
Locus ID 54207
Cytogenetics 14q31.3
Protein Families WB
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Other "KCNK10" proteins (5)
SKU Description Size Price
LC403348 Lyophilized KCNK10 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC408699 Lyophilized KCNK10 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC412052 Lyophilized KCNK10 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403348 Transient overexpression lysate of potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY408699 Transient overexpression lysate of potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3 (as WB positive control) 100 ug
$436.00

file-alt Citations

Up-Regulation of TREK-2 Potassium Channels in Cultured Astrocytes Requires De Novo Protein Synthesis: Relevance to Localization of TREK-2 Channels in Astrocytes after Transient Cerebral Ischemia
Rivera-Pagán, Rivera-Aponte, Melnik-Martínez et al
PLoS ONE (2015) 10 (4), e0125195 DOI: 10.1371/journal.pone.0125195
Cited by 12
AI Summary: OriGene’s TREK-2 positive control lysate (cat. no. LY412052) was used in this study to validate the identity of the TREK-2 protein band detected by Western blot, confirming the up-regulation of TREK-2 channels in astrocytes after ischemic conditions.
Snippet: TREK-2 was detected as a band of around 60kDa which is consistent with the predicted molecular weight of 59.6kDa. In some experiments, a positive control (Origene, cat. no. LY412052) was used to validate the identity of the band. Cell surface biotinylation. ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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