ZNF350 (NM_021632) Human Over-expression Lysate
SKU
LY411959
Transient overexpression lysate of zinc finger protein 350 (ZNF350) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC201006 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_021632, NP_067645 |
| Synonyms | ZBRK1; ZFQR |
| Predicted MW | 60 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_067645 |
| Locus ID | 59348 |
| Cytogenetics | 19q13.41 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "ZNF350" proteins (2)
Citations
ZNF350 promoter methylation accelerates colon cancer cell migration
Tanaka, Kuwano, Nishikawa et al
Oncotarget (2018) 9 (95), 36750-36769 DOI: 10.18632/oncotarget.26353
Tanaka, Kuwano, Nishikawa et al
Oncotarget (2018) 9 (95), 36750-36769 DOI: 10.18632/oncotarget.26353
Snippet: Furthermore, we examined ZNF350 expression in cDNAs prepared from paired normal and tumor tissues of 22 patients with colon adenocarcinoma (HCRT103 TissueScan qPCR Arrays;OriGene Technologies, Rockville, MD, USA), and found that colon cancer tissues have significantly reduced ZNF350 mRNA expression when compared with the surrounding normal colon tissues (Figure (Figure6C).6C). However, there was no significant difference in relative changes in ZNF350 mRNA levels (cancer/normal) between stages I/II and stages III/IV colon cancers (Figure (Figure6D).6D)
Showing 1-1 of 1 papers.
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