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PVRIG (NM_024070) Human Over-expression Lysate

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SKU
LY411386
Transient overexpression lysate of poliovirus receptor related immunoglobulin domain containing (PVRIG) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210470
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_024070, NP_076975
Synonyms C7orf15; CD112R
Predicted MW 34.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_076975
Locus ID 79037
Cytogenetics 7q22.1
Protein Families WB
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Other "PVRIG" proteins (1)
SKU Description Size Price
LC411386 Lyophilized PVRIG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Translational plasticity facilitates the accumulation of nonsense genetic variants in the human population
Jagannathan, Bradley
Genome Res. (2016) 26 (12), 1639-1650 DOI: 10.1101/gr.205070.116
Cited by 42
AI Summary: OriGene’s PVRIG lysate (LY411386) was used in this study to determine the specificity of antibodies in Western blotting experiments.
Snippet: ...prebound to 10-50 microg of antibody (PVRIG: ab168153 [Abcam...described below using the same antibodies used for the immunoprecipitation...lysate of PVRIG (LY411386;Origene Technologies) served as a...determine the specificity of the antibodies used for Western blotting...
Showing 1-1 of 1 papers.
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