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C11orf49 (NM_024113) Human Over-expression Lysate

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SKU
LY411347
Transient overexpression lysate of chromosome 11 open reading frame 49 (C11orf49), transcript variant 3 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201435
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_024113, NP_077018
Predicted MW 37.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_077018
Locus ID 79096
Cytogenetics 11p11.2
Protein Families WB
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Other "C11orf49" proteins (11)
SKU Description Size Price
PH301435 C11orf49 MS Standard C13 and N15-labeled recombinant protein (NP_077018) 10 ug
$3,360.00
LC411347 Lyophilized C11orf49 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424096 Lyophilized C11orf49 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424097 Lyophilized C11orf49 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424098 Lyophilized C11orf49 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY424096 Transient overexpression lysate of chromosome 11 open reading frame 49 (C11orf49), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY424097 Transient overexpression lysate of chromosome 11 open reading frame 49 (C11orf49), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY424098 Transient overexpression lysate of chromosome 11 open reading frame 49 (C11orf49), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP301435 Recombinant protein of human chromosome 11 open reading frame 49 (C11orf49), transcript variant 3, 20 µg 20 ug
$867.00
TP760102 Recombinant protein of human chromosome 11 open reading frame 49 (C11orf49), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00
TP761377 Purified recombinant protein of Human chromosome 11 open reading frame 49 (C11orf49), transcript variant 2, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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