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Plexin A2 (PLXNA2) (NM_025179) Human Over-expression Lysate

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SKU
LY410858
Transient overexpression lysate of plexin A2 (PLXNA2) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221024
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_025179, NP_079455
Synonyms OCT; PLXN2
Predicted MW 210.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_079455
Locus ID 5362
Cytogenetics 1q32.2
Protein Families WB
Protein Pathways Axon guidance
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Documents
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Other "Plexin A2" proteins (1)
SKU Description Size Price
LC410858 Lyophilized PLXNA2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Fyn-dependent phosphorylation of PlexinA1 and PlexinA2 at conserved tyrosines is essential for zebrafish eye development
St Clair, Emerson, D'Elia et al
FEBS J. (2017)
Cited by 21
AI Summary: OriGene’s human PLXNA2 plasmid (pCMV6-Entry with C-terminal Myc and Flag tag) was used in this study to express wild-type and mutant PLXNA2 constructs, enabling analysis of Fyn-dependent phosphorylation and its role in zebrafish eye development.
Snippet: 4.2. Plasmids, Cell Culture, and Transfections The human PLXNA2 construct (Origene Technologies, Rockville, MD, USA) harbored human PLXNA2 (NP_079455) containing a C-terminal Myc and Flag tag in pCMV6-Entry. The cloning sites were SgfI and MluI
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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