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HSD3B7 (NM_025193) Human Over-expression Lysate

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SKU
LY410844
Transient overexpression lysate of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202468
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_025193, NP_079469
Synonyms CBAS1; PFIC4; SDR11E3
Predicted MW 41 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_079469
Locus ID 80270
Cytogenetics 16p11.2
Protein Families WB
Protein Pathways Metabolic pathways, Primary bile acid biosynthesis
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Documents
Resources
Other "HSD3B7" proteins (1)
SKU Description Size Price
LC410844 Lyophilized HSD3B7 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

3β-Isoobeticholic acid efficiently activates the farnesoid X receptor (FXR) due to its epimerization to 3α-epimer by hepatic metabolism
Stefela, Kaspar, Drastik et al
J. Steroid Biochem. Mol. Biol. (2020) 202, 105702 DOI: 10.1016/j.jsbmb.2020.105702
Cited by 7
AI Summary: OriGene contributed to this study by providing the HSD3B7 (Hydroxy-Delta-5-Steroid Dehydrogenase) expression construct, which was used to investigate the enzymatic conversion of bile acid derivatives relevant to FXR activation.
Snippet: The Hydroxy-Delta-5-Steroid Dehydrogenase (syn. 3-Beta-Hydroxy-Delta(5)-C27 Steroid Oxidoreductase, HSD3B7) expression construct was purchased from Origene (Rockville, MD, USA)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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