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DNAJC5B (NM_033105) Human Over-expression Lysate

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SKU
LY409749
Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily C, member 5 beta (DNAJC5B) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205251
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_033105, NP_149096
Synonyms CSP-beta
Predicted MW 22.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_149096
Locus ID 85479
Cytogenetics 8q13.1
Protein Families WB
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Other "DNAJC5B" proteins (3)
SKU Description Size Price
PH305251 DNAJC5B MS Standard C13 and N15-labeled recombinant protein (NP_149096) 10 ug
$3,360.00
LC409749 Lyophilized DNAJC5B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305251 Recombinant protein of human DnaJ (Hsp40) homolog, subfamily C, member 5 beta (DNAJC5B), 20 µg 20 ug
$867.00

file-alt Citations

Heat shock proteins HSPB8 and DNAJC5B have HCV antiviral activity
Braga, Carneiro, Batista et al
PLoS ONE (2017) 12 (11), e0188467 DOI: 10.1371/journal.pone.0188467
AI Summary: In this study, the pCMV6/DNAJC5B expression vector from OriGene Technologies was used to increase DNAJC5B gene expression, enabling the researchers to assess its antiviral effect against hepatitis C virus.
Snippet: Gene expression vectors. To increase the expression of the target genes, the expression vectors pcDNA3.1/HSPB8 (Thermo Fisher Scientific, CA, USA) and pCMV6/DNAJC5B (OriGene Technologies, USA) were used. As controls, the empty expression vectors were Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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