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ABHD16B (NM_080622) Human Over-expression Lysate

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SKU
LY409161
Transient overexpression lysate of chromosome 20 open reading frame 135 (C20orf135) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211836
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080622, NP_542189
Synonyms C20orf135; dJ591C20.1
Predicted MW 52.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_542189
Locus ID 140701
Cytogenetics 20q13.33
Protein Families WB
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Documents
Resources
Other "ABHD16B" proteins (1)
SKU Description Size Price
LC409161 Lyophilized ABHD16B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Association of α/β-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition
Shan, Xu, Bleyer et al
Int J Mol Sci (2020) 21 (2) DOI: 10.3390/ijms21020627
Cited by 23
AI Summary: OriGene’s Human ABHD16B over-expression lysate (NM_080622) was used as a positive control in Western blotting experiments to detect ABHD16B protein expression in bovine tissues in this study.
Snippet: …Human ABHD16B over-expression lysate (NM_080622, OriGene, Rockville, MD, USA) was used as a positive control. …Human ABHD16B over-expression lysate (NM_080622, OriGene, Rockville, MD, USA) was used as a positive control. …
Showing 1-1 of 1 papers.
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