LRPPRC (NM_133259) Human Over-expression Lysate
SKU
LY408850
Transient overexpression lysate of leucine-rich PPR-motif containing (LRPPRC) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC216747 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_133259, NP_573566 |
| Synonyms | CLONE-23970; GP130; LRP130; LSFC; MC4DN5 |
| Predicted MW | 157.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_573566 |
| Locus ID | 10128 |
| Cytogenetics | 2p21 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "LRPPRC" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH316747 | LRPPRC MS Standard C13 and N15-labeled recombinant protein (NP_573566) | 10 ug |
$3,360.00
|
|
| LC408850 | Lyophilized LRPPRC HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP316747 | Recombinant protein of human leucine-rich PPR-motif containing (LRPPRC), 20 µg | 20 ug |
$737.00
|
|
Citations
Knockdown of the cellular protein LRPPRC attenuates HIV-1 infection
Schweitzer, Matthews, Madson et al
PLoS ONE (2012) 7 (7), e40537 DOI: 10.1371/journal.pone.0040537
Schweitzer, Matthews, Madson et al
PLoS ONE (2012) 7 (7), e40537 DOI: 10.1371/journal.pone.0040537
Cited by 21
AI Summary: OriGene products, specifically the shLRPPRC 02, 03, 04 shRNA plasmids, the non-specific (NS) shRNA plasmid, and the pFlag-LRPPRC plasmid, were used to manipulate LRPPRC expression in cell lines, enabling the study of its role in HIV-1 infection.
Snippet: LRPPRC (shLRPPRC 02, 03, 04), the non-specific (NS) shRNA plasmid, and pFlag-LRPPRC were obtained from Origene Technologies (Rockville ... the requirement of LRPPRC for HIV-1 infection, we constructed 293T cell lines stably depleted of LRPPRC by short hairpin RNA
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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