ROBO1 (NM_133631) Human Over-expression Lysate

CAT#: LY408772

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Transient overexpression lysate of roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2



  View other "ROBO1" proteins (7)

LY408772 is the same product as LY430029.

USD 495.00


Availability*
5 Days

Size
    • 100 ug


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Specifications

Product Data
Description Transient overexpression lysate of roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211961
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Accession Number NM_133631, NP_598334
Other Names DUTT1; SAX3
Predicted MW 175.7 kDa
Components 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage The lysate is shipped with dry ice. The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
Reference Data
RefSeq NP_598334
Locus ID 6091
Cytogenetics 3p12.3
Protein Families Druggable Genome
Protein Pathways Axon guidance

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