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CMTM2 (NM_144673) Human Over-expression Lysate

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SKU
LY408212
Transient overexpression lysate of CKLF-like MARVEL transmembrane domain containing 2 (CMTM2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206884
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_144673, NP_653274
Synonyms CKLFSF2
Predicted MW 27.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_653274
Locus ID 146225
Cytogenetics 16q21
Protein Families WB
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Documents
Resources
Other "CMTM2" proteins (1)
SKU Description Size Price
LC408212 Lyophilized CMTM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Systematic investigation of CMTM family genes suggests relevance to glioblastoma pathogenesis and CMTM1 and CMTM3 as priority targets
Delic, Thuy, Schulze et al
Genes Chromosomes Cancer (2015) 54 (7), 433-43 DOI: 10.1002/gcc.22255
Cited by 38
AI Summary: In this study, an OriGene CMTM2 plasmid was used to overexpress CMTM2 in glioblastoma cells, enabling the authors to assess its functional impact on tumor cell behavior.
Snippet: 70%. Overexpression of CMTM2 and 5. The plasmid encoding CMTM5 was purchased from GE-Healthcare Bio-Sciences (Piscataway, NJ) and the plasmid encoding CMTM2 was purchased from ORIGENE (Rockville, MD). The ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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