Dysadherin (FXYD5) (NM_144779) Human Over-expression Lysate
SKU
LY408093
Transient overexpression lysate of FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1 (as WB positive control)
- LY408093 is the same product as LY430120.
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203917 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_144779, NP_659003 |
| Synonyms | DYSAD; HSPC113; IWU1; KCT1; OIT2; PRO6241; RIC |
| Predicted MW | 19.5 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_659003 |
| Locus ID | 53827 |
| Cytogenetics | 19q13.12 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
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Resources
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Other "Dysadherin" proteins (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC402284 | Lyophilized FXYD5 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC408093 | Lyophilized FXYD5 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC431776 | Lyophilized FXYD5 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402284 | Transient overexpression lysate of FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY431776 | Transient overexpression lysate of FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| TP761830 | Purified recombinant protein of Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
Citations
High-Intensity Training Represses FXYD5 and Glycosylates Na,K-ATPase in Type II Muscle Fibres, Which Are Linked with Improved Muscle K+ Handling and Performance
Hostrup, Lemminger, Thomsen et al
Int J Mol Sci (2023) 24 (6) DOI: 10.3390/ijms24065587
Hostrup, Lemminger, Thomsen et al
Int J Mol Sci (2023) 24 (6) DOI: 10.3390/ijms24065587
Cited by 12
AI Summary: OriGene’s FXYD5 overexpression (OE) cell lysates (catalog LY408093) were used as positive controls to validate antibody specificity for FXYD5 detection in human skeletal muscle samples in this study.
Snippet: ogy, Dallas, TX, USA). Both antibodies showed multiple signals from 19 to 40 kDa in human skeletal muscle homogenates and FXYD5 overexpression (OE) cell lysates compared to the control (C) (LY408093, OriGene Technologies, Rockville, MD, USA), indicating that the abs recognise FXYD5 (Figure 5B,C). Afterwards, we tested the effect of the amount of protein loaded. With a low amount of protein loaded
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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