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APOL3 (NM_145640) Human Over-expression Lysate

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SKU
LY407940
Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant alpha/d (as WB positive control)
  • LY407940 is the same product as LY430136.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220066
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_145640, NP_663615
Synonyms apoL-III; APOLIII; CG12_1; CG121
Predicted MW 44.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_663615
Locus ID 80833
Cytogenetics 22q12.3
Protein Families WB
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Other "APOL3" proteins (15)
SKU Description Size Price
PH308643 APOL3 MS Standard C13 and N15-labeled recombinant protein (NP_663614) 10 ug
$3,360.00
LC403433 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407916 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407939 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407940 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC410766 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC415350 Lyophilized APOL3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403433 Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant beta/a (as WB positive control) 100 ug
$436.00
LY407916 Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant beta/b (as WB positive control) 100 ug
$436.00
LY407939 Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant alpha/c (as WB positive control) 100 ug
$436.00
LY410766 Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant alpha/b (as WB positive control) 100 ug
$436.00
LY415350 Transient overexpression lysate of apolipoprotein L, 3 (APOL3), transcript variant alpha/a (as WB positive control) 100 ug
$436.00
TP308643 Recombinant protein of human apolipoprotein L, 3 (APOL3), transcript variant alpha/c, 20 µg 20 ug
$867.00
TP761559 Purified recombinant protein of Human apolipoprotein L, 3 (APOL3), transcript variant beta/a, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00
TP762662 Purified recombinant protein of Human apolipoprotein L, 3 (APOL3), transcript variant beta/b, full length, with N-GST and C-His tag, expressed in E.coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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