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Cathepsin B (CTSB) (NM_147782) Human Over-expression Lysate

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SKU
LY407802
Transient overexpression lysate of cathepsin B (CTSB), transcript variant 4 (as WB positive control)
  • LY407802 is the same product as LY430184.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
In Stock*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222463
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_147782, NP_680092
Synonyms APPS; CPSB; RECEUP
Predicted MW 37.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_680092
Locus ID 1508
Cytogenetics 8p23.1
Protein Families WB
Protein Pathways Antigen processing and presentation, Lysosome
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Other "Cathepsin B" proteins (20)
SKU Description Size Price
PH310578 CTSB MS Standard C13 and N15-labeled recombinant protein (NP_680093) 10 ug
$3,360.00
PH322289 CTSB MS Standard C13 and N15-labeled recombinant protein (NP_001899) 10 ug
$3,360.00
PH322463 CTSB MS Standard C13 and N15-labeled recombinant protein (NP_680092) 10 ug
$3,360.00
PH322764 CTSB MS Standard C13 and N15-labeled recombinant protein (NP_680091) 10 ug
$3,360.00
LC403447 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407800 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407801 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407802 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC419666 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430182 Lyophilized CTSB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403447 Transient overexpression lysate of cathepsin B (CTSB), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY407800 Transient overexpression lysate of cathepsin B (CTSB), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY407801 Transient overexpression lysate of cathepsin B (CTSB), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY419666 Transient overexpression lysate of cathepsin B (CTSB), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY430182 Transient overexpression lysate of cathepsin B (CTSB), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP310578 Recombinant protein of human cathepsin B (CTSB), transcript variant 5, 20 µg 20 ug
$867.00
TP322289 Recombinant protein of human cathepsin B (CTSB), transcript variant 1, 20 µg 20 ug
$867.00
TP322463 Purified recombinant protein of Homo sapiens cathepsin B (CTSB), transcript variant 4, 20 µg 20 ug
$867.00
TP322764 Purified recombinant protein of Homo sapiens cathepsin B (CTSB), transcript variant 3, 20 µg 20 ug
$867.00
TP720340 Recombinant protein of human cathepsin B (CTSB), transcript variant 1 10 ug
$340.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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