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IL4I1 (NM_172374) Human Over-expression Lysate

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SKU
LY406700
Transient overexpression lysate of interleukin 4 induced 1 (IL4I1), transcript variant 2 (as WB positive control)
  $665.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221972
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_172374, NP_758962
Synonyms FIG1; hIL4I1; LAAO; LAO
Predicted MW 65.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_758962
Locus ID 259307
Cytogenetics 19q13.33
Protein Families WB
Protein Pathways Alanine, aspartate and glutamate metabolism, Cysteine and methionine metabolism, leucine and isoleucine degradation, Metabolic pathways, Phenylalanine, Phenylalanine metabolism, Tryptophan metabolism, tyrosine and tryptophan biosynthesis, Tyrosine metabolism, Valine
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Other "IL4I1" proteins (4)
SKU Description Size Price
PH321972 IL4I1 MS Standard C13 and N15-labeled recombinant protein (NP_758962) 10 ug
$3,360.00
LC406700 Lyophilized IL4I1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP321972 Recombinant protein of human interleukin 4 induced 1 (IL4I1), transcript variant 2, 20 µg 20 ug
$867.00
TP701092 Purified recombinant protein of Human interleukin 4 induced 1 (IL4I1), Gln22-End, with C-terminal His tag, secretory expressed in HEK293 cells, 50 ug 50 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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