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TTLL6 (NM_173623) Human Over-expression Lysate

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SKU
LY406512
Transient overexpression lysate of tubulin tyrosine ligase-like family, member 6 (TTLL6), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207279
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_173623, NP_775894
Synonyms TTL.6
Predicted MW 64.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_775894
Locus ID 284076
Cytogenetics 17q21.32
Protein Families WB
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Documents
Resources
Other "TTLL6" proteins (1)
SKU Description Size Price
LC406512 Lyophilized TTLL6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Amyloid-β oligomers induce synaptic damage via Tau-dependent microtubule severing by TTLL6 and spastin
Zempel, Luedtke, Kumar et al
EMBO J. (2013)
Cited by 286
AI Summary: OriGene’s TTLL6-tGFP plasmid was used as a source to generate TTLL6-YFP constructs, enabling the study of TTLL6’s role in microtubule dynamics and synaptic damage in the context of Alzheimer’s disease.
Snippet: All RNAi sequences were cloned into a pShuttle-mRFP/mTFP or pSuper vector. TTLL6-tGFP was obtained from Origene and recloned to TTLL6-YFP
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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