PPP2R2B (NM_181674) Human Over-expression Lysate

SKU
LY405663
Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224533
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_181674, NP_858060
Synonyms B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12
Predicted MW 51.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_858060
Locus ID 5521
Cytogenetics 5q32
Protein Families WB
Protein Pathways Tight junction
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Other "PPP2R2B" proteins (15)
SKU Description Size Price
LC405663 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405664 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405665 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405666 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405667 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417895 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426765 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429210 Lyophilized PPP2R2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY405664 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY405665 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 4 (as WB positive control) 100 ug
$665.00
LY405666 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 5 (as WB positive control) 100 ug
$665.00
LY405667 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 6 (as WB positive control) 100 ug
$665.00
LY417895 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY426765 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 7 (as WB positive control) 100 ug
$436.00
LY429210 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform (PPP2R2B), transcript variant 1 (as WB positive control) 100 ug
$436.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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