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FAM36A (COX20) (NM_198076) Human Over-expression Lysate

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SKU
LY405090
Transient overexpression lysate of family with sequence similarity 36, member A (FAM36A) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217381
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198076, NP_932342
Synonyms FAM36A; MC4DN11
Predicted MW 13.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_932342
Locus ID 116228
Cytogenetics 1q44
Protein Families WB
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Other "FAM36A" proteins (3)
SKU Description Size Price
PH317381 FAM36A MS Standard C13 and N15-labeled recombinant protein (NP_932342) 10 ug
$3,360.00
LC405090 Lyophilized COX20 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP317381 Recombinant protein of human family with sequence similarity 36, member A (FAM36A), 20 µg 20 ug
$867.00

file-alt Citations

Identification and Characterization of the Role of REEP5 in Sarco-Endoplasmic Reticulum Formation, Maintenance, and Function in Cardiac Muscle
Lee
Thesis (2021)
Snippet: … Immunodepletion experiments were performed by incubating primary antibodies with 10 μg of purified cMyc/DDK-tagged human FAM162A (Origene, LY415331), cMyc/DDKtagged human MCT1 (Origene, LY401069), or cMyc/DDK-tagged human COX20 (Origene, LY405090) lysates in 10 mL of 5% milk (1μg /mL) for 3 hr at 4°C prior to probing the membrane at 4°C overnight
Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium
Lee, Hadipour-Lakmehsari, Kim et al
Sci Data (2020) 7 (1), 425 DOI: 10.1038/s41597-020-00762-1
Cited by 20
AI Summary: OriGene products—cMyc/DDK-tagged human FAM162A (LY415331), MCT1 (LY401069), and COX20 (LY405090)—were used as purified protein lysates for immunodepletion experiments to validate antibody specificity in the study.
Snippet: pped immunoblots are available and provided as Supplemental Fig. 9. Immunodepletion experiments were performed by incubating primary antibodies with 10 μg of purified cMyc/DDK-tagged human FAM162A (Origene, LY415331), cMyc/DDK-tagged human MCT1 (Origene, LY401069), or cMyc/DDK-tagged human COX20 (Origene, LY405090) lysates in 10 mL of 5% milk (1μg /mL) for 3 hr at 4°C prior to probing the
FigureFigureFigure
Showing 1-2 of 2 papers.
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