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Ribonuclease A (RNASE1) (NM_198232) Human Over-expression Lysate

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SKU
LY404956
Transient overexpression lysate of ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 3 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217926
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198232, NP_937875
Synonyms RAC1; RIB1; RNS1
Predicted MW 17.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_937875
Locus ID 6035
Cytogenetics 14q11.2
Protein Families WB
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Other "Ribonuclease A" proteins (10)
SKU Description Size Price
PH305620 RNASE1 MS Standard C13 and N15-labeled recombinant protein (NP_937878) 10 ug
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LC401024 Lyophilized RNASE1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC404956 Lyophilized RNASE1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404957 Lyophilized RNASE1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404958 Lyophilized RNASE1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401024 Transient overexpression lysate of ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY404957 Transient overexpression lysate of ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY404958 Transient overexpression lysate of ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP305620 Recombinant protein of human ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 1, 20 µg 20 ug
$737.00
TP720748 Purified recombinant protein of Human ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), transcript variant 4 10 ug
$340.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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