OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Spastin (SPAST) (NM_199436) Human Over-expression Lysate

Be the first to review this product
SKU
LY404560
Transient overexpression lysate of spastin (SPAST), transcript variant 2 (as WB positive control)
  $665.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA351199 Rabbit Polyclonal Anti-SPAST Antibody
100 ul
$205.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Spastin or NM_199436"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214100
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_199436, NP_955468
Synonyms ADPSP; FSP2; SPG4
Predicted MW 63.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_955468
Locus ID 6683
Cytogenetics 2p22.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Spastin" proteins (5)
SKU Description Size Price
PH314100 SPAST MS Standard C13 and N15-labeled recombinant protein (NP_955468) 10 ug
$3,360.00
PH320458 SPAST MS Standard C13 and N15-labeled recombinant protein (NP_055761) 10 ug
$3,360.00
LC404560 Lyophilized SPAST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP314100 Recombinant protein of human spastin (SPAST), transcript variant 2, 20 µg 20 ug
$867.00
TP320458 Recombinant protein of human spastin (SPAST), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Gene dosage-dependent rescue of HSP neurite defects in SPG4 patients' neurons
Havlicek, Kohl, Mishra et al
Hum Mol Genet (2014) 23 (10), 2527-41 DOI: 10.1093/hmg/ddt644
Cited by 126
AI Summary: In this study, OriGene’s TrueORF cDNA clones for human SPAST (RC208065 for M1 isoform, RC208066 for M87 isoform) were used to overexpress spastin isoforms in SPG4 patient-derived neurons, demonstrating that increased spastin dosage can rescue neurite defects associated with hereditary spastic paraplegia.
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products