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RNF214 (NM_207343) Human Over-expression Lysate

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SKU
LY403969
Transient overexpression lysate of ring finger protein 214 (RNF214), transcript variant 1 (as WB positive control)
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219941
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_207343, NP_997226
Predicted MW 77.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_997226
Locus ID 257160
Cytogenetics 11q23.3
Protein Families WB
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Other "RNF214" proteins (3)
SKU Description Size Price
LC403969 Lyophilized RNF214 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421388 Lyophilized RNF214 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY421388 Transient overexpression lysate of ring finger protein 214 (RNF214), transcript variant 2 (as WB positive control) 100 ug
$665.00

file-alt Citations

Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays
Forsström, Bisławska Axnas, Stengele et al
Mol. Cell Proteomics (2014)
AI Summary: OriGene products, specifically the recombinant proteins MKNK2 (LY413712), RNF214 (LY403969), GPR56 (LY428998), PRY2 (LY424151), CDK2AP2 (LY417039), MS4A8B (LY403116), and STARD13 (LY406044), were used in this study to validate antibody binding and specificity observed in peptide array experiments.
Snippet: MKNK2 LY413712, RNF214 LY403969, GPR56 LY428998, PRY2 LY424151, CDK2AP2 LY417039, MS4A8B LY403116, STARD13 LY406044, all from Origene, Rockville, MD) were run on precast 4-20% CriterionTM TGXTM SDS-PAGE gradient gels (Bio-Rad ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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