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PKC beta 1 (PRKCB) (NM_212535) Human Over-expression Lysate

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SKU
LY403957
Transient overexpression lysate of protein kinase C, beta (PRKCB), transcript variant 1 (as WB positive control)
  • LY403957 is the same product as LY431009.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220599
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_212535, NP_997700
Synonyms PKC-beta; PKCB; PKCbeta; PKCI(2); PRKCB1; PRKCB2
Predicted MW 76.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_997700
Locus ID 5579
Cytogenetics 16p12.2-p12.1
Protein Families WB
Protein Pathways B cell receptor signaling pathway, Calcium signaling pathway, Chemokine signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway
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Other "PKC beta 1" proteins (7)
SKU Description Size Price
PH307217 PRKCB MS Standard C13 and N15-labeled recombinant protein (NP_002729) 10 ug
$3,360.00
PH320599 PRKCB MS Standard C13 and N15-labeled recombinant protein (NP_997700) 10 ug
$3,360.00
LC403957 Lyophilized PRKCB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC419133 Lyophilized PRKCB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY419133 Transient overexpression lysate of protein kinase C, beta (PRKCB), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP307217 Recombinant protein of human protein kinase C, beta (PRKCB), transcript variant 2, 20 µg 20 ug
$867.00
TP320599 Recombinant protein of human protein kinase C, beta (PRKCB), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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