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GAB2 (NM_080491) Human Over-expression Lysate

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SKU
LY403310
Transient overexpression lysate of GRB2-associated binding protein 2 (GAB2), transcript variant 1 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215435
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080491, NP_536739
Predicted MW 74.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_536739
Locus ID 9846
Cytogenetics 11q14.1
Protein Families WB
Protein Pathways Chronic myeloid leukemia, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis
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Other "GAB2" proteins (3)
SKU Description Size Price
LC403310 Lyophilized GAB2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC415859 Lyophilized GAB2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY415859 Transient overexpression lysate of GRB2-associated binding protein 2 (GAB2), transcript variant 2 (as WB positive control) 100 ug
$665.00

file-alt Citations

Elevated Gab2 induces tumor growth and angiogenesis in colorectal cancer through upregulating VEGF levels
Ding, Luo, Fan et al
J. Exp. Clin. Cancer Res. (2017) 36 (1), 56 DOI: 10.1186/s13046-017-0524-2
AI Summary: OriGene's Gab2 antibody was used for immunohistochemistry analysis to detect Gab2 protein expression levels in colorectal cancer tissue samples in this study.
Snippet: For the immunohistochemistry, we performed as previously described [26, 27]. The Gab2 (OriGene Technologies, USA) and VEGF (Abcam, UK) primary antibodies were used at a 1:150 dilution in the immunohistochemistry analysis. ... All 5 versions Cite SaveSaving
FigureFigureFigure
IHC
Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Ding, Luo, Li et al
J. Exp. Clin. Cancer Res. (2016) 35 (1), 5 DOI: 10.1186/s13046-015-0280-0
Cited by 71
AI Summary: OriGene’s Gab2 antibody was used in this study to detect and analyze Gab2 protein expression, helping to elucidate its role in promoting epithelial-to-mesenchymal transition and metastasis in colorectal cancer.
Snippet: The following commercial antibodies were used in this study: Gab2 (OriGene Technologies, USA), E-cadherin, vimentin, MMP7 and MMP9 (Abcam, UK), phospho-AKT, total AKT, phospho-ERK1/2 and total ERK1/2 (Invitrogen, USA), GAPDH and β-actin (Immunology ... Cite SaveSaving
FigureFigureFigure
WB
Showing 1-2 of 2 papers.
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