OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TWIST2 (NM_057179) Human Over-expression Lysate

Be the first to review this product
SKU
LY403298
Transient overexpression lysate of twist homolog 2 (Drosophila) (TWIST2) (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

Frequently Bought Together (2)

TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "TWIST2 or NM_057179"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205006
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_057179, NP_476527
Synonyms AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS
Predicted MW 18.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_476527
Locus ID 117581
Cytogenetics 2q37.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "TWIST2" proteins (3)
SKU Description Size Price
PH305006 TWIST2 MS Standard C13 and N15-labeled recombinant protein (NP_476527) 10 ug
$3,360.00
LC403298 Lyophilized TWIST2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305006 Recombinant protein of human twist homolog 2 (Drosophila) (TWIST2), 20 µg 20 ug
$867.00

file-alt Citations

The emerging role of Twist proteins in hematopoietic cells and hematological malignancies
Merindol, Riquet, Szablewski et al
Blood Cancer J (2014) 4, e206 DOI: 10.1038/bcj.2014.22
Cited by 38
AI Summary: OriGene’s Lymphoma cDNA Array II (LYRT102) was used in this study to perform real-time Q-PCR analysis on lymphoma samples, enabling investigation of Twist1–2 expression in B-cell lymphoma.
Snippet: With the aim of investigating a possible role of Twist1" style="font-weight: bold;">Twist1–2 in B-cell lymphoma, we performed real-time Q-PCR on samples derived from Origene (Lymphoma cDNA Array II LYRT102, OriGene Technologies, Inc., Rockville, MD, USA) (Figure 3). A description of each sample ... Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products