CREB3L1 (NM_052854) Human Over-expression Lysate

SKU
LY403260
Transient overexpression lysate of cAMP responsive element binding protein 3-like 1 (CREB3L1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC204968
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_052854, NP_443086
Synonyms OASIS; OI16
Predicted MW 57 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_443086
Locus ID 90993
Cytogenetics 11p11.2
Protein Families WB
Protein Pathways Huntington's disease, Melanogenesis, Prostate cancer
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Other "CREB3L1" proteins (2)
SKU Description Size Price
LC403260 Lyophilized CREB3L1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP760562 Purified recombinant protein of Human cAMP responsive element binding protein 3-like 1 (CREB3L1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
García, Torres Demichelis, Viale et al
J. Cell. Sci. (2017)
Cited by 42
AI Summary: OriGene’s pGFP-C-shLenti vector was used to inhibit CREB3L1 expression, enabling the authors to investigate CREB3L1’s role in regulating secretory pathway adaptation in hormone-stimulated thyroid cells.
Snippet: (Tree Star, Ashland, OR). Journal of Cell Science • Accepted manuscript Page 20. ShRNA We inhibited CREB3L1 expression using a commercial pGFP-C-shLenti vector (Origene, Rockville, MD). This vector was designed to inhibit CREB3L1 expression
Showing 1-1 of 1 papers.
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