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ORAI1 (NM_032790) Human Over-expression Lysate

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SKU
LY403208
Transient overexpression lysate of ORAI calcium release-activated calcium modulator 1 (ORAI1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210034
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032790, NP_116179
Synonyms CRACM1; IMD9; ORAT1; TAM2; TMEM142A
Predicted MW 32.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_116179
Locus ID 84876
Cytogenetics 12q24.31
Protein Families WB
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Other "ORAI1" proteins (1)
SKU Description Size Price
LC403208 Lyophilized ORAI1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Selective activation of the transcription factor NFAT1 by calcium microdomains near Ca2+ release-activated Ca2+ (CRAC) channels
Kar, Nelson, Parekh
J. Biol. Chem. (2011) 286 (17), 14795-803 DOI: 10.1074/jbc.M111.220582
Cited by 24
AI Summary: OriGene contributed to this study by providing the Orai1 plasmid, which was used to investigate the role of CRAC channels in NFAT1 activation and nuclear translocation.
Snippet: Orai1 was from OriGene, and STIM1-YFP was a generous gift from Dr. Tobias Meyer (Stanford University). ... 2+ oscillations in the presence of CRAC channel blockers (7). However, these oscillations are similar to those obtained in Ca 2+ -free solution and run down more quickly
Showing 1-1 of 1 papers.
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