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CARD11 (NM_032415) Human Over-expression Lysate

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SKU
LY403162
Transient overexpression lysate of caspase recruitment domain family, member 11 (CARD11) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222740
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032415, NP_115791
Synonyms BENTA; BIMP3; CARMA1; IMD11; IMD11A; PPBL
Predicted MW 133.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_115791
Locus ID 84433
Cytogenetics 7p22.2
Protein Families WB
Protein Pathways B cell receptor signaling pathway, T cell receptor signaling pathway
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Other "CARD11" proteins (3)
SKU Description Size Price
PH322740 CARD11 MS Standard C13 and N15-labeled recombinant protein (NP_115791) 10 ug
$3,360.00
LC403162 Lyophilized CARD11 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP322740 Purified recombinant protein of Homo sapiens caspase recruitment domain family, member 11 (CARD11), 20 µg 20 ug
$737.00

file-alt Citations

Novel CARD11 Mutations in Human Cutaneous Squamous Cell Carcinoma Lead to Aberrant NF-κB Regulation
Watt, Purdie, den Breems et al
Am. J. Pathol. (2015)
Cited by 28
AI Summary: OriGene’s anti-DDK antibody (TA50011) was used to analyze CARD11 protein expression by immunoblotting in this study investigating the role of CARD11 mutations in cutaneous squamous cell carcinoma.
Snippet: Extract Kit (Active Motif). Protein concentration was determined by the Bradford assay and CARD11 expression was analyzed by immunoblotting with an anti-DDK antibody (TA50011;Origene, Rockville, MD). Wild-type CARD11 ... Cite SaveSaving
WB
Showing 1-1 of 1 papers.
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