C2orf88 (NM_032321) Human Over-expression Lysate

SKU
LY403155
Transient overexpression lysate of chromosome 2 open reading frame 88 (C2orf88), transcript variant 4 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213211
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032321, NP_115697
Synonyms smAKAP
Predicted MW 10.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_115697
Locus ID 84281
Cytogenetics 2q32.2
Protein Families WB
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LY420957 Transient overexpression lysate of chromosome 2 open reading frame 88 (C2orf88), transcript variant 1 (as WB positive control) 100 ug
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LY420958 Transient overexpression lysate of chromosome 2 open reading frame 88 (C2orf88), transcript variant 3 (as WB positive control) 100 ug
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LY420959 Transient overexpression lysate of chromosome 2 open reading frame 88 (C2orf88), transcript variant 2 (as WB positive control) 100 ug
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TP302987 Recombinant protein of human hypothetical protein MGC13057 (MGC13057), transcript variant 1, 20 µg 20 ug
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TP313211 Recombinant protein of human hypothetical protein MGC13057 (MGC13057), transcript variant 4, 20 µg 20 ug
$867.00
TP313362 Purified recombinant protein of Homo sapiens hypothetical protein MGC13057 (MGC13057), transcript variant 3, 20 µg 20 ug
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TP324360 Purified recombinant protein of Homo sapiens hypothetical protein MGC13057 (MGC13057), transcript variant 2, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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