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WNT2B (NM_024494) Human Over-expression Lysate

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SKU
LY402992
Transient overexpression lysate of wingless-type MMTV integration site family, member 2B (WNT2B), transcript variant WNT-2B2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213949
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_024494, NP_078613
Synonyms WNT13
Predicted MW 43.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_078613
Locus ID 7482
Cytogenetics 1p13.2
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
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Other "WNT2B" proteins (5)
SKU Description Size Price
LC401347 Lyophilized WNT2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC402992 Lyophilized WNT2B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401347 Transient overexpression lysate of wingless-type MMTV integration site family, member 2B (WNT2B), transcript variant WNT-2B1 (as WB positive control) 100 ug
$436.00
TP762265 Purified recombinant protein of Human wingless-type MMTV integration site family, member 2B (WNT2B), transcript variant WNT-2B1, Tyr250-Glu350, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00
TP762623 Purified recombinant protein of Human wingless-type MMTV integration site family, member 2B (WNT2B), transcript variant WNT-2B1, full length, with N-terminal GST and C-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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