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LGI2 (NM_018176) Human Over-expression Lysate

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SKU
LY402669
Transient overexpression lysate of leucine-rich repeat LGI family, member 2 (LGI2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211015
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018176, NP_060646
Synonyms LGIL2
Predicted MW 62.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_060646
Locus ID 55203
Cytogenetics 4p15.2
Protein Families WB
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Other "LGI2" proteins (1)
SKU Description Size Price
LC402669 Lyophilized LGI2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Similarity of molecular phenotype between known epilepsy gene LGI1 and disease candidate gene LGI2
Limviphuvadh, Chua, Rahim et al
BMC Biochem (2010) 11, 39 DOI: 10.1186/1471-2091-11-39
Cited by 6
AI Summary: In this study, OriGene products, specifically the LGI1 (RC211489) and LGI2 (RC219973) cDNA clones, were used to express wild-type and mutant proteins, enabling the authors to analyze their secretion efficiency and subcellular localization in investigating the molecular similarities between LGI1 and LGI2 in epilepsy.
FigureFigureFigure
Showing 1-1 of 1 papers.
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