OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

NADPH oxidase 4 (NOX4) (NM_016931) Human Over-expression Lysate

Be the first to review this product
SKU
LY402584
Transient overexpression lysate of NADPH oxidase 4 (NOX4), transcript variant 1 (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA349083 Rabbit Polyclonal Anti-NOX4 Antibody
100 ug
$640.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "NADPH oxidase 4 or NM_016931"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208007
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_016931, NP_058627
Synonyms KOX; KOX-1; RENOX
Predicted MW 66.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_058627
Locus ID 50507
Cytogenetics 11q14.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "NADPH oxidase 4" proteins (1)
SKU Description Size Price
LC402584 Lyophilized NOX4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

NADPH oxidase 1 is highly expressed in human large and small bowel cancers
Lu, Jiang, Wu et al
PLoS ONE (2020) 15 (5), e0233208 DOI: 10.1371/journal.pone.0233208
Cited by 29
AI Summary: OriGene products, specifically the Myc-DDK-tagged-NOX2 plasmid (#RC208997) and pCMV-MycDDK-HsNOX4 plasmid (#RC208007), were used to generate HEK293 cell clones overexpressing NOX2 and NOX4, which facilitated the validation of the novel NOX1 antibody in this study.
Snippet: The HEK293-NOX2 clone was obtained by transfection of Myc-DDK-tagged-NOX2 plasmid (4 μg) (#RC208997, Origene, Rockville, MD), and the HEK293-NOX4 clone was obtained by transfection of pCMV-MycDDK-HsNOX4 plasmid (4 μg) (#RC208007, Origene, Rockville Cached
NOX isoforms in the development of abdominal aortic aneurysm
Siu, Li, Zhang et al
Redox Biol (2016) 11, 118-125 DOI: 10.1016/j.redox.2016.11.002
AI Summary: OriGene contributed to this study by providing the full-length coding region of human NOX4 (NM_016931, isoform A) in pCMV6-hNOX4, which was used to investigate the role of NOX4 in abdominal aortic aneurysm development.
Snippet: Full length coding region of human NOX4 (NM_016931, isoform A of human NOX4) in pCMV6-hNOX4 was purchased from OriGene (Rockville, MD, USA)
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products