NADPH oxidase 4 (NOX4) Rabbit Polyclonal Antibody

Snippet: … GKT137831 (20 μM, Selleck, 1218942-37-0), an inhibitor of NOX4, was used to inhibit the activity of NOX4 in NRCMs78,79. For RNA interference, cells were transfected with FGFR3 siRNA (Origene, SR513507) or control scrambled siRNA (Origene, SR30004) by Lipofectamine 3000 for 12 h in Opti-MEM. After the transfection, cells were removed to a full-growth medium for another 12 h and;- For RNA interference, cells were transfected with FGFR3 siRNA (Origene, SR513507) or control scrambled siRNA (Origene, SR30004) by Lipofectamine 3000 for 12 h in

Snippet: 9348) and GAPDH (CST, 5174), Lamin B (CST, 12,586), Keap1 (CST, 4678), c-Caspase-3 (CST, 9661), HDAC3 (CST, 85057), eNOS (CST, 32027), acetylated-lysine (CST, 9441), 3-NT (Millipore, 05–233), Nox4 (Origene, TA349083). Assay of eNOS dimer/monomer SDS-resistant eNOS dimers and monomers were assayed by using low-temperature SDS-PAGE as described previously [40]. Briefly, after being washed twice w

Snippet: 4. Materials and Methods. 4.1. Antibodies and Reagents. The antibodies against NOX4, P38, JNK, OPN, MCP-1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from OriGene Technologies (Rockville, MD, USA) Cached;

Snippet: Science). Immunodetection was performed as described previously (48) using as primary antibody either anti-NOX4 (OriGene);anti- pSmad3 (ser 423) (Abcam);anti-Smad3 (Abcam);anti-p22phox (Santa Cruz Biotechnology Inc.), anti-NIS (Thermoscientific);anti- ... Cite SaveSaving