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VRL1 (TRPV2) (NM_016113) Human Over-expression Lysate

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SKU
LY402501
Transient overexpression lysate of transient receptor potential cation channel, subfamily V, member 2 (TRPV2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202821
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_016113, NP_057197
Synonyms VRL; VRL-1; VRL1
Predicted MW 85.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_057197
Locus ID 51393
Cytogenetics 17p11.2
Protein Families WB
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Other "VRL1" proteins (1)
SKU Description Size Price
LC402501 Lyophilized TRPV2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

TRPV2 is a novel biomarker and therapeutic target in triple negative breast cancer
Elbaz, Ahirwar, Xiaoli et al
Oncotarget (2018) 9 (71), 33459-33470 DOI: 10.18632/oncotarget.9663
Snippet: Full length TRPV2 overexpressing plasmid has been purchased from (Origene). In addition, small interfering RNA (SiRNA) either non-targeting or TRPV2 Si-RNA have been purchased from (Dharmacon) company. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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