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SIRT3 (NM_012239) Human Over-expression Lysate

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SKU
LY402174
Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) (SIRT3), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200190
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_012239, NP_036371
Synonyms SIR2L3
Predicted MW 43.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_036371
Locus ID 23410
Cytogenetics 11p15.5
Protein Families WB
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Other "SIRT3" proteins (5)
SKU Description Size Price
LC402174 Lyophilized SIRT3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422739 Lyophilized SIRT3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422739 Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) (SIRT3), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP760441 Purified recombinant protein of Human sirtuin 3 (SIRT3), nuclear gene encoding mitochondrial protein, transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00
TP761108 Purified recombinant protein of Human sirtuin 3 (SIRT3), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Honokiol Inhibits Atrial Metabolic Remodeling in Atrial Fibrillation Through Sirt3 Pathway
Liu, Xu, Zang et al
Front Pharmacol (2022) 13, 813272 DOI: 10.3389/fphar.2022.813272
Cited by 24
AI Summary: In this study, OriGene products including Sirt3 overexpression plasmid (NM_022433) and Sirt3 siRNA (SR501803) were used to manipulate Sirt3 expression in HL-1 cells, enabling the authors to investigate the role of Sirt3 in honokiol-mediated inhibition of atrial metabolic remodeling in atrial fibrillation.
FigureFigureFigure
Mitochondrial complex II is a source of the reserve respiratory capacity that is regulated by metabolic sensors and promotes cell survival
Pfleger, He, Abdellatif
Cell Death Dis (2015) 6, e1835 DOI: 10.1038/cddis.2015.202
Cited by 265
AI Summary: OriGene products contributed to this study by providing human cDNA clones for Pdk1 (SC321678/NM_002610), Pdk4 (SC118542/NM_002612), Sdhaf1 (NM_001042631), and Sirt3 (NM_012239), which were used to investigate the regulation of mitochondrial reserve respiratory capacity and cell survival.
Snippet: The human cDNA clones for Pdk1 (SC321678/NM_002610), Pdk4 (SC118542/NM_002612), Sdhaf1 (NM_001042631) and Sirt3 (NM_012239) were purchased (Origene Technologies, Inc., Rockville, MD, USA) and cloned into recombinant adenovirus vectors, ... Cite SaveSaving
Showing 1-2 of 2 papers.
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