SIRT1 (NM_012238) Human Over-expression Lysate
SKU
LY402173
Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (SIRT1), transcript variant 1 (as WB positive control)
$665.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC218134 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_012238, NP_036370 |
| Synonyms | SIR2; SIR2alpha; SIR2L1 |
| Predicted MW | 81.5 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_036370 |
| Locus ID | 23411 |
| Cytogenetics | 10q21.3 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SIRT1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH318134 | SIRT1 MS Standard C13 and N15-labeled recombinant protein (NP_036370) | 10 ug |
$3,360.00
|
|
| LC402173 | Lyophilized SIRT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP318134 | Recombinant protein of human sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (SIRT1), transcript variant 1, 20 µg | 20 ug |
$737.00
|
|
Citations
SIRT1 affects DNA methylation of polycomb group protein target genes, a hotspot of the epigenetic shift observed in ageing
Wakeling, Ions, Escolme et al
Hum Genomics (2015) 9 (1), 14 DOI: 10.1186/s40246-015-0036-0
Wakeling, Ions, Escolme et al
Hum Genomics (2015) 9 (1), 14 DOI: 10.1186/s40246-015-0036-0
Cited by 22
AI Summary: In this study, OriGene products—specifically SIRT1 expression plasmids and SIRT1 siRNA—were used to manipulate SIRT1 levels in human cell lines, enabling the authors to investigate how SIRT1 influences DNA methylation at polycomb group protein target genes.
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity
Rahman, Nirala, Singh et al
J. Cell Biol. (2014) 206 (2), 289-305 DOI: 10.1083/jcb.201404118
Rahman, Nirala, Singh et al
J. Cell Biol. (2014) 206 (2), 289-305 DOI: 10.1083/jcb.201404118
Cited by 116
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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